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Life Science Technician 2
The Chen lab (http://www.xiaokechenlab.com/) has a position for a motivated research assistant at the Life Science Technician II level with strong interests in molecular and circuitry neuroscience. Our lab focuses on understanding the circuitry and molecular basis for learning, drug addiction and chronic pain by using various viral tracing, circuitry manipulation, in vivo imaging/recording methods. This position will provide opportunities to learn a range of cellular and molecular techniques and offers a unique opportunity to collaborate with physiological and molecular neuroscientists.

The main responsibilities of the successful candidate will be performing cell culture, producing and purifying adeno-associated viruses. Other responsibilities will include performing routine laboratory techniques such as genotyping, PCRs and cloning, as well as maintaining records of lab work, curating samples and assisting in data analysis.
Characteristic Duties:
The duties listed under this section are designed to provide a representative sampling of key tasks and/or responsibilities associated with the job. They are not intended to be a complete list of all the duties performed by employees in the classification.

• Perform animal surgical techniques such as suturing, catheterization and infusion of arteries, small organ dissection, organ perfusion and tumor implantation and passage in small animals.
• Immunohistological procedures including frozen sectioning and multi-step immunologic staining for fluorescence or enzymatic detection. Some level of histologic assessment may be required.
• Maintenance, transfer and establishment of cell lines from normal or tumor cells, preparation and bioassay of serum, screening for contamination, isolation of cell mutants, cell staining, mass production of cells and cell cloning.
• Fixation, dehydration, staining and embedding of specimens for electron microscopy; carbon coating, negative staining, shadowing, operation of electron microscope (EM) either transmission or scanning; use of an ultramicrotome with adiamond knife for routine thin sectioning; alignment of an EM where the system is automated.
• Purification and /or structural characterization of compounds for which a protocolis well established.
• Separation of cells, organelles, or molecules using density gradients.
• Radioimmunoassay of viruses, proteins, or hormone levels in cell extract according to established protocol.
• Techniques such as electrophoresis, chromatography, autoradiography, radiolabeling of animals, cell cultures, or macromolecules.
• Isolation, purification and maintenance of mammalian viruses and bacteriophages; determination of viral concentration.
• Genetic manipulation of bacteria including mutagenesis, ‘phage transduction and transformation.
• Accurate documentation of experimental protocols and basic statistical analysis of data.

Minimum Education and Experience Required
Two years of laboratory experience or two years of college coursework in the scientific discipline a combination of equivalent education and relevant work experience.
Minimum Qualifications
Work at the Life Science Technician II level requires experience and ability beyond that required for the Life Science Technician I, including increased technical skills and problem-solving abilities
Physical Requirements
• Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand.
• Ability to stand and move on hard surfaces for up to eight hours.
• Occasionally lift and handle materials up to 10 pounds
• Rarely carry/pull and/or push up to 20 pounds
• Must have correctible vision to perform duties of the job.•Position may require repetitive motion
Working Conditions
Work occurs in a research laboratory environment. Depending on research, may work in the vicinity of animals, chemicals, gasses, or hazardous materials. Expected to follow all safety requirements and procedures including wearing appropriate Personal Protective Equipment (PPE) if required
Work Standards
Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.
Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University’s Administrative Guide, http://adminguide.stanford.edu/.and the appropriate collective bargaining agreement at https://cardinalatwork.stanford.edu/hr-processes-policiespolicies/labor-relations-collective-bargaining.
Why Stanford is for You
Imagine a world without search engines or social platforms. Consider lives saved through first-ever organ transplants and research to cure illnesses. Stanford University has revolutionized the way we live and enrich the world. Supporting this mission is our diverse and dedicated 17,000 staff. We seek talent driven to impact the future of our legacy. Our culture and unique perks empower you with:
• Freedom to grow. We offer career development programs, tuition reimbursement, or audit a course. Join a TedTalk, film screening, or listen to a renowned author or global leader speak. • A caring culture. We provide superb retirement plans, generous time-off, and family care resources. • A healthier you. Climb our rock wall, or choose from hundreds of health or fitness classes at our world-class exercise facilities. We also provide excellent health care benefits. • Discovery and fun. Stroll through historic sculptures, trails, and museums. • Enviable resources. Enjoy free commuter programs, ridesharing incentives, discounts and more

Stanford is an equal employment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law. Stanford welcomes applications from all who would bring additional dimensions to the University’s research, teaching and clinical missions.

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