Research Scientist: Exosome & Nanotherapy

<a target="_blank" href="http://www.pandorumtechnologies.com/">Pandorum Technologies</a> is an innovation driven deep-tech start-up, in the field of Tissue Engineering and Regenerative Medicine, with operations in India and the USA. Pandorum uses its proprietary technology platform to develop advanced therapeutic formulations consisting of cells, cell modulators and biopolymers for regenerative treatment of human tissue. Pandorum is currently moving into clinical translation, with two pipelines - Regenerative treatment for i) Corneal blindness and ii) Acute Lung Injury. Medical University of South Carolina (MUSC), at Charleston, is a key centre for our R&D with translation focus, with clinical studies planned to commence in 2021. Although our primary targets are Cornea and Lung regeneration, the tissue agnostic platform core allows Pandorum to develop advanced regenerative therapeutics beyond individual targets, opening avenues to multi-organ regeneration through cellular reprogramming. Pandorum has recently raised <a target="_blank" href="https://inc42.com/buzz/exclusive-biotech-startup-pandorum-technologies-raises-over-inr-41-cr/">fresh funds</a>, been recognized as one of the <a target="_blank" href="https://www.youtube.com/watch?v=_ovJYtcFefg">Top 10 Biotech Start-ups of India 2020</a>, and climbing higher as you read this!

Check out our detailed job description below to know more about us-

Starting date: Immediate Start.

Place of work: Medical University of South Carolina, Charleston (the picturesque city in the East Coast US, known for the attractions like The Battery, Rainbow Row and more).

Responsibilities:

This position within Nanotherapy group would involve:

1. Performing experiments involving stem cell culture and sub-culture processes. The candidate will be expected to have a solid background in mammalian stem cell biology. Experience of processing human, rabbit and murine tissues, extract cells from primary origin and establish culture would be preferred.
2. The work will involve understanding how different culture protocols involving nutritional, chemical, physical inducers may affect stem cell expression profile.
3. Perform cell culture, genomic analysis, RT PCR, ELISA assays, histology, flow cytometry and other molecular bench work with thorough knowledge of Cell based assays, various microscopic techniques including confocal studies and Electron Microscopy.
4. Assist R&D scientists in the design, production, and characterization of exosomes including but not limited to transient transfection, chromatographic purification, and affinity characterization by flow cytometry, vFC or cell binding and uptake assays.
5. Prior knowledge of Lipid Nanoparticle based macromolecular cargo delivery and cellular uptake analysis would be helpful.
6. Characterize scaffold expression and binding affinity by a variety of assays including interferometry, SPR, and vesicle flow cytometry would be preferred.
7. Experience in handling and dosing experimental animals (rodents) for all major routes of administration and dosing will be a plus.
8. Purification of cell derived secretome, exosomes for multiple biomarkers- including RNA and protein based biomarker characterization will be a key undertaken.
9. Cell based functional assays based on anti-inflammatory, anti-fibrotic, anti/ pro-angiogenic, cell migration (scratch) and re-innervation assays will be a key undertaken.
10. Adhere to SOPs, study protocols and study reports to ensure GLP-like studies are performed.

Roles and Required Skills will include:

• Regularly exercise technical discretion in design, execution and interpretation of experiments that contribute to project goals. Select methods and techniques for obtaining solutions within broadly defined practices and procedures
• Prepare technical reports, summaries, protocols and quantitative analyses. Communicate these results regularly at team meetings.
• Manage laboratory resources, ordering reagents and other lab supplies as needed.
• Make detailed observations, analyse data, and interpret results. Summarize experimental data timely and assist in drafting figures, results, and methods for publication under the direction of the PI.
• Acquire a thorough understanding of the present scientific literature relating to the research in the laboratory.
• The candidate should have good publication record, Author and contribute to development reports, scientific publications, patents, and regulatory submissions.
• Coordinate tasks across multiple projects, demonstrating prioritization and planning. We are a start-up and are looking for applicants who are self-motivated critical thinkers driven to meet project timelines and willing to adapt in a highly dynamic, collaborative and cross-disciplinary environment.


Qualifications:


Essential:
Associate Research Scientist: Masters (MS, MSc, M-Tech) plus minimum three years of experience.

Research Scientist: A PhD in Cellular Biology, Molecular Biology, Nanotechnology, Biomedical Engineering or a related field.

Laboratory Skills: Must have hands-on experience with mammalian cell culture, flow cytometry, and PCR (real time, RT) analysis. Expertise with immunohistochemistry, histology, microscopy, small animal procedures, mouse models, ELISA assays and other molecular biology assays would be crucial. Candidates with prior experience in stem cell biology, stem cell based secreted cell modulators including exosomes and bioprocessing will be preferred.

Desirable: Candidates with prior experience in stem cell biology, stem cell based secreted cell modulators including exosomes and bioprocessing will be preferred.

Ideal Candidate:
We are a fast-moving start-up primed for exponential growth and look forward to working with the best minds that can push the frontiers of product development using regenerative medicine tools.
An ideal candidate would be a quick-learner, with excellent communication skills, a passion to build cutting edge products who is able to follow new protocols, generate new knowledge, provide suggestions for improvement and multi-task experiments in a fast-paced environment. Additionally, the ideal candidate should have an understanding of good laboratory practices, superior problem-solving skills, have wonderful team dynamics ability and an independent worker.

If you are interested in joining a vibrant company, then please email us with your CV and a cover letter to: contact@pandorumtechnologies.in